SubtiBank SubtiBank
yfjN [2018-01-02 12:12:56]
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yfjN [2018-01-02 12:12:56]

putative tRNA-dihydrouridine synthase 2
Locus
BSU08030
Isoelectric point
5.38
Molecular weight
36.83 kDa
Protein length
325 aa Sequence Blast
Gene length
975 bp Sequence Blast
Function
tRNA modification
Product
putative tRNA-dihydrouridine synthase 2
Essential
no
Synonyms
dusC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

    • Gene

    Coordinates
    876,426 → 877,403

    The protein

    Catalyzed reaction/ biological activity

  • a uridine in tRNA + NADPH + H+ → a 5,6-dihydrouridine in tRNA + NADP+

    • Protein family

  • dus family (according to Swiss-Prot)

    • Paralogous protein(s)

  • YacF

    • Cofactors

  • FMN or FAD PubMed

    • Expression and Regulation

    Operons

    Genes
    yfjN
    Description
    PubMed

    Regulation

  • the leader mRNA is processed by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed
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    Biological materials

    Mutant

  • MGNA-C277 (yfjN::erm), available at the NBRP B. subtilis, Japan
  • BKE08030 (ΔyfjN::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACAAAAATCAAATCCT, downstream forward: _UP4_TAACAGCAGTGCTTGATTCA
  • BKK08030 (ΔyfjN::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACAAAAATCAAATCCT, downstream forward: _UP4_TAACAGCAGTGCTTGATTCA

    • References

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