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yfjN [2018-01-02 12:12:56]
putative tRNA-dihydrouridine synthase 2
function
tRNA modification
product
putative tRNA-dihydrouridine synthase 2
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]Gene
Coordinates
876,426 → 877,403
The protein
Catalyzed reaction/ biological activity
a uridine in tRNA + NADPH + H+ → a 5,6-dihydrouridine in tRNA + NADP+Protein family
dus family (according to Swiss-Prot)Paralogous protein(s)
[protein|CE45D56BC413555AF4E7914B5307AD967A358452|YacF][SW|Cofactors]
FMN or FAD [Pubmed|21635694]Expression and Regulation
Operons
genes
[gene|B1C2BD8CEEABF9BBAE2646E8E24457A485097CFC|yfjN]
description
[Pubmed|29794222]
regulation
the leader mRNA is processed by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]view in new tabBiological materials
Mutant
MGNA-C277 (yfjN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2275 NBRP B. subtilis, Japan]BKE08030 (Δ[gene|B1C2BD8CEEABF9BBAE2646E8E24457A485097CFC|yfjN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAAAAATCAAATCCT, downstream forward: _UP4_TAACAGCAGTGCTTGATTCABKK08030 (Δ[gene|B1C2BD8CEEABF9BBAE2646E8E24457A485097CFC|yfjN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAAAAATCAAATCCT, downstream forward: _UP4_TAACAGCAGTGCTTGATTCAReferences